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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
18/11/2016 |
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Data da última atualização: |
10/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HONNA, P. T.; FUGANTI-PAGLIARINI, R.; FERREIRA, L. C.; MOLINARI, M. D. C.; MARIN, S. R. R.; OLIVEIRA, M. C. N. de; FARIAS, J. R. B.; NEUMAIER, N.; MERTZ-HENNING, L. M.; KANAMORI, N.; NAKASHIMA, K.; TAKASAKI, H.; URANO, K.; SHINOZAKI, K.; YAMAGUCHI-SHINOZAKI, K.; DESIDÉRIO, J. A.; NEPOMUCENO, A. L. |
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Afiliação: |
PATRICIA T. HONNA, UNESP; RENATA FUGANTI PAGLIARINI, CAPES; LEONARDO C. FERREIRA, CNPq; MAYLA D. C. MOLINARI, UEL; SILVANA REGINA ROCKENBACH MARIN, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; NORMAN NEUMAIER, CNPSO; LILIANE MARCIA MERTZ HENNING, CNPSO; NORIHITO KANAMORI, JIRCAS; KAZUO NAKASHIMA, JIRCAS; HIRONORI TAKASAKI, RIKEN Center for Sustainable Resource Science; KAORU URANO, RIKEN Center for Sustainable Resource Science; KAZUO SHINOZAKI, RIKEN Center for Sustainable Resource Science; KAZUKO YAMAGUCHI-SHINOZAKI, University of Tokyo; JANETE A. DESIDÉRIO, UNESP Jaboticabal; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Molecular Breeding, v. 36, n. 11, nov. 2016. |
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ISSN: |
1572-9788 |
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DOI: |
10.1007/s11032-016-0570-z |
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Idioma: |
Inglês |
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Conteúdo: |
Water deficit may occur at any stage of crop development, affecting productivity and causing economic losses. In response to drought, raffinose family oligosaccharides (RFOs) are accumulated in plant tissues stabilizing and protecting cell membranes and keeping the vital functions. The enzyme galactinol synthase (GolS, EC 2.4.1.123) catalyzes the first step in the biosynthesis of RFOs. In our study, soybean events overexpressing 35S:AtGolS2 were molecularly, physiological, and agronomical characterized, under drought simulated in greenhouse and in field conditions during the crop season 2014/2015. The conventional soybean cultivar BRS 184 was transformed and five positive events were obtained. Four events transmitted the transgene to further generations and in the events 2Ia1 and 2Ia4, two to four copies of AtGols2 gene were observed. Results in greenhouse showed that the overexpression of AtGolS2 in genetically modified (GM) plants led to increased galactinol transcripts, probably resulting in changes in carbohydrate metabolism. Accumulation of these transcripts that may have acted as osmoprotectors, lead to higher drought tolerance and survival rate of 2Ia4 plants. In addition, in field conditions, higher yield was observed for 2Ia4 plants under irrigated (IRR) and non-irrigated (NIRR) treatments. This result can be due to the increased synthesis of RFOs even under well-watered conditions. This field screening showed promising results for drought tolerance, suggesting that 2Ia4 plants may be useful in a breeding program for the development of drought-tolerant plants. However, additional studies are needed in further crop seasons and other sites to better characterize how these plants may outperform the WT plants under water deficit. MenosWater deficit may occur at any stage of crop development, affecting productivity and causing economic losses. In response to drought, raffinose family oligosaccharides (RFOs) are accumulated in plant tissues stabilizing and protecting cell membranes and keeping the vital functions. The enzyme galactinol synthase (GolS, EC 2.4.1.123) catalyzes the first step in the biosynthesis of RFOs. In our study, soybean events overexpressing 35S:AtGolS2 were molecularly, physiological, and agronomical characterized, under drought simulated in greenhouse and in field conditions during the crop season 2014/2015. The conventional soybean cultivar BRS 184 was transformed and five positive events were obtained. Four events transmitted the transgene to further generations and in the events 2Ia1 and 2Ia4, two to four copies of AtGols2 gene were observed. Results in greenhouse showed that the overexpression of AtGolS2 in genetically modified (GM) plants led to increased galactinol transcripts, probably resulting in changes in carbohydrate metabolism. Accumulation of these transcripts that may have acted as osmoprotectors, lead to higher drought tolerance and survival rate of 2Ia4 plants. In addition, in field conditions, higher yield was observed for 2Ia4 plants under irrigated (IRR) and non-irrigated (NIRR) treatments. This result can be due to the increased synthesis of RFOs even under well-watered conditions. This field screening showed promising results for drought tolerance, suggesting that... Mostrar Tudo |
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Palavras-Chave: |
Estresse abiótico. |
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Thesagro: |
Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02888naa a2200361 a 4500
001 2056622
005 2017-07-10
008 2016 bl uuuu u00u1 u #d
022 $a1572-9788
024 7 $a10.1007/s11032-016-0570-z$2DOI
100 1 $aHONNA, P. T.
245 $aMolecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance.$h[electronic resource]
260 $c2016
520 $aWater deficit may occur at any stage of crop development, affecting productivity and causing economic losses. In response to drought, raffinose family oligosaccharides (RFOs) are accumulated in plant tissues stabilizing and protecting cell membranes and keeping the vital functions. The enzyme galactinol synthase (GolS, EC 2.4.1.123) catalyzes the first step in the biosynthesis of RFOs. In our study, soybean events overexpressing 35S:AtGolS2 were molecularly, physiological, and agronomical characterized, under drought simulated in greenhouse and in field conditions during the crop season 2014/2015. The conventional soybean cultivar BRS 184 was transformed and five positive events were obtained. Four events transmitted the transgene to further generations and in the events 2Ia1 and 2Ia4, two to four copies of AtGols2 gene were observed. Results in greenhouse showed that the overexpression of AtGolS2 in genetically modified (GM) plants led to increased galactinol transcripts, probably resulting in changes in carbohydrate metabolism. Accumulation of these transcripts that may have acted as osmoprotectors, lead to higher drought tolerance and survival rate of 2Ia4 plants. In addition, in field conditions, higher yield was observed for 2Ia4 plants under irrigated (IRR) and non-irrigated (NIRR) treatments. This result can be due to the increased synthesis of RFOs even under well-watered conditions. This field screening showed promising results for drought tolerance, suggesting that 2Ia4 plants may be useful in a breeding program for the development of drought-tolerant plants. However, additional studies are needed in further crop seasons and other sites to better characterize how these plants may outperform the WT plants under water deficit.
650 $aSoja
653 $aEstresse abiótico
700 1 $aFUGANTI-PAGLIARINI, R.
700 1 $aFERREIRA, L. C.
700 1 $aMOLINARI, M. D. C.
700 1 $aMARIN, S. R. R.
700 1 $aOLIVEIRA, M. C. N. de
700 1 $aFARIAS, J. R. B.
700 1 $aNEUMAIER, N.
700 1 $aMERTZ-HENNING, L. M.
700 1 $aKANAMORI, N.
700 1 $aNAKASHIMA, K.
700 1 $aTAKASAKI, H.
700 1 $aURANO, K.
700 1 $aSHINOZAKI, K.
700 1 $aYAMAGUCHI-SHINOZAKI, K.
700 1 $aDESIDÉRIO, J. A.
700 1 $aNEPOMUCENO, A. L.
773 $tMolecular Breeding$gv. 36, n. 11, nov. 2016.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
02/07/2013 |
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Data da última atualização: |
02/07/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KELLERMANN, B.; LACERDA, A. E. B. de; ROSOT, M. A. D. |
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Afiliação: |
BETINA KELLERMANN; ANDRE EDUARDO BISCAIA DE LACERDA, CNPF; MARIA AUGUSTA DOETZER ROSOT, CNPF. |
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Título: |
Bamboo influence on natural regeneration - a case study in a Araucaria Forest fragment in Brazil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: CONGRESO LATINOAMERICANO DE IUFRO, 3., 2013, San José. Bosques, competitividad y territorios sostenibles. San José: CATIE; [S.l.]: IUFRO, 2013. |
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Páginas: |
1 p. |
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Idioma: |
Inglês |
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Notas: |
IUFROLAT 2013. Disponibilizado online. Resumen. |
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Palavras-Chave: |
Diversidade; Planta invasora; Sucessão ecológica. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/85262/1/Bamboo.pdf
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Marc: |
LEADER 00706nam a2200181 a 4500
001 1961130
005 2013-07-02
008 2013 bl uuuu u00u1 u #d
100 1 $aKELLERMANN, B.
245 $aBamboo influence on natural regeneration - a case study in a Araucaria Forest fragment in Brazil.$h[electronic resource]
260 $aIn: CONGRESO LATINOAMERICANO DE IUFRO, 3., 2013, San José. Bosques, competitividad y territorios sostenibles. San José: CATIE; [S.l.]: IUFRO$c2013
300 $a1 p.
500 $aIUFROLAT 2013. Disponibilizado online. Resumen.
653 $aDiversidade
653 $aPlanta invasora
653 $aSucessão ecológica
700 1 $aLACERDA, A. E. B. de
700 1 $aROSOT, M. A. D.
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